Referenced in: none

Automatically associated channels: Kv2.1

Title: Single-channel currents underlying glycinergic inhibitory postsynaptic responses in spinal neurons.

Authors: T Takahashi, A Momiyama

Journal, date & volume: Neuron, 1991 Dec , 7, 965-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/1722412

Abstract
Single-channel properties of glycine receptors have been characterized so far only in cultured neurons. To characterize the glycine receptor channels in situ, we applied the patch-clamp technique to spinal neurons in slice preparations. Glycine-gated, single-channel currents were recorded in outside-out patches excised from spinal neurons. In the falling phase of glycinergic inhibitory synaptic currents, single-channel currents were resolved as discrete steps. In both cases, the glycine-gated channels showed similar multiple conductance levels. These results suggest that the receptor channel properties are indistinguishable in the synaptic and extrasynaptic sites. We conclude that multiple conductance states of a receptor channel are the native feature of the glycine receptor in situ.