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CHO

Description: Chinese Hamster Ovary Cells

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Introduction

CHO cells are a line of epithelial-like cells that was initiated in 1957 by T.T. Puck and colleagues at the Department of Biophysics, University of Colorado Medical School in Denver, USA from a biopsy of an ovary of an adult Chinese hamster [1352]. There are several specific features that make the CHOcell line one of the most convenient solutions for reconstitution of mammalian ionchannels and related proteins: 1) it is a mammalian cell line; 2) they are adherent cells that make them easier to transfect compared to cell lines that grow in suspension; 3) cells are not electrotonically coupled and so are easy to voltage clamp, and 4) they express very little endogenous currents. [1353]


Experimental data

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Ion channels

TRPM4, KCNK5, Kir, Neglegible Ik.


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References

Yarishkin OV et al. Endogenous TRPM4-like channel in Chinese hamster ovary (CHO) cells.
Biochem. Biophys. Res. Commun., 2008 May 2 , 369 (712-7).

Beattie KA et al. Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics.
J. Physiol. (Lond.), 2018 05 15 , 596 (1813-1828).


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Credits

To cite this page: [Contributors] Channelpedia https://channelpedia.epfl.ch/wikipages/239/ , accessed on 2024 Dec 12



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