Channelpedia

BKβ3

Description: potassium large conductance calcium-activated channel, subfamily M beta member 3
Gene: Kcnmb3
Alias: kcnmb3, BKB3

Edit - History

Introduction

Until 1999, only one beta subunit of BK (MaxiK) channels was known at the molecular level. This subunit (now known as the Beta-1-subunit) accounted mainly for MaxiK properties in vascular smooth muscle cells (VSMCs), where it is highly expressed. In the following years, three more beta subunits have been cloned and characterized. (Orio [540])

KCNMB3 (also known as HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3) encodes BKB3, potassium large conductance calcium-activated channel (subfamily M) beta member 3. MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. BKB3 may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22.

http://www.ncbi.nlm.nih.gov/gene/27094


Edit - History

Gene

The b3-subunit (BKB3) was cloned from human EST databases and is phylogenetically more related to b2 than to b1 (Uebele [1179]). There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. (Orio [540])

The mouse beta3 subunit, excluding N-terminal splice variants, shares only 62.8% amino acid identity with its human counterpart. (Zheng [1184])

Species NCBI gene ID Chromosome Position
Human 27094 3 27347
Mouse 100502876 3 38050
Rat 310303 2 14354

Edit

Transcript

Species NCBI accession Length (nt)
Human NM_171828.3 1307
Mouse NM_001195074.1 720
Rat NM_001104560.2 1637

Edit

Protein Isoforms

Species Uniprot ID Length (aa)
Human Q9NPA1 279
Mouse E9Q7U0 239
Rat A7VL23 239

Isoforms

Transcript
Length (nt)
Protein
Length (aa)
Variant
Isoform

Edit

Post-Translational Modifications

PTM
Position
Type

Edit - History

Structure

The topology of alpha and beta subunits that make up BK channels can be seen in fig. 1 of Orio [540]. The channel is formed by 4 alpha-subunits and probably 4 beta subunits. Regulatory beta subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular “loop” and with NH2 and COOH terminals oriented toward the cytoplasm (Fig. 1 [540]). The loop has three or four putative glycosylation sites. Four beta subunits have been cloned in mammals (Brenner [1181], Knaus [1167], Uebele [1179], Xia [1182]).

BKB3 is phylogenetically more related to BKB2 than to BKB1 (Uebele [1179]). There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. (Orio [540])

BKβ3 predicted AlphaFold size

Species Area (Å2) Reference
Human 2573.02 source
Mouse 2618.57 source
Rat 2709.57 source

Methodology for AlphaFold size prediction and disclaimer are available here


Edit - History

Expression and Distribution

BKB3 was detected in testis, pancreas and spleen (Xia [1182]).


Edit - History

Function

There are four splice variants (BKB3a-d), whose differences are in the NH2-terminal region. Each splice variant confers different inactivation properties to the MaxiK channel. Whereas the BKB3a and BKB3c subunits confer similar inactivation properties, BKB3b induces a faster and incomplete inactivation process that becomes evi- dent only at large depolarizations (Xia [1182]). It is unclear whether or not the BKB3d-subunit interacts with the alpha-subunit since coexpression of alpha- and BKB3d-subunits does not produce changes in the Ca2+ activation curves or in the gating kinetics of the MaxiK channel. (Orio [540])

The BKB3b auxiliary subunit, when coexpressed with the Slo alpha subunit, results in a particularly rapid (1‬ms), but incomplete inactivation, mediated by the cytosolic NH2 terminus of the BKB3b subunit (Xia et al., 2000 [1182]). See Lingle [1186] for by a two-step blocking mechanism of this process.


References

540

Orio P et al. New disguises for an old channel: MaxiK channel beta-subunits.
News Physiol. Sci., 2002 Aug , 17 (156-61).


Edit

Credits

To cite this page: [Contributors] Channelpedia https://channelpedia.epfl.ch/wikipages/74/ , accessed on 2024 Dec 12



Add section