Referenced in: none

Automatically associated channels: Kir6.2 , Slo1

Title: Postnatal induction and neural regulation of inward rectifiers in mouse skeletal muscle.

Authors: T Gonoi, S Hasegawa

Journal, date & volume: Pflugers Arch., 1991 Jul , 418, 601-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/1719474

Abstract
The whole-cell voltage-clamp technique was used to examine developmental changes of inward rectifier currents in fibres of the flexor digitorum brevis muscle acutely isolated from mice on postnatal day 0 (P0) to P36. Neither a steady-state component (Is-s) nor a slowly activated component (Irise) of inward rectifier currents were observed in fibres of P0 and P4 mice. Both Is-s and Irise became apparent between days P8 and P16. The specific amplitudes of Is-s and Irise measured at a test-pulse potential of -100 mV at 20 mM extracellular K+ [( K+]o) increased to their respective platcau values of -68 +/- 10 and -15 +/- 7 microA/cm2 at P20. In fibres denervated on day P4 the developmental increase of Is-s was suppressed, its specific amplitude at P20 being one-tenth of that in the corresponding normal fibres. Irise did not appear in P4-denervated fibres throughout the development. In muscle fibres denervated at P16 or P20, the specific amplitudes of Is-s and Irise decreased, reaching the levels of P4-denervated fibres in 2-4 days after denervation. We conclude that Is-s and Irise develop within 3 weeks after birth, and suggest that innervation plays a key role in their induction.