Referenced in: none

Automatically associated channels: Kir2.3

Title: A molecular scheme for the reaction between gamma-aminobutyric acid and the most abundant chloride channel on crayfish deep extensor abdominal muscle.

Authors: H Adelsberger, N von Beckerath, F Parzefall, J Dudel

Journal, date & volume: Pflugers Arch., 1996 Mar , 431, 680-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8596717

Abstract
Single-channel measurements were performed with the aim of constructing a detailed molecular scheme for the reaction between gamma-aminobutyric acid (GABA) and a chloride channel of crayfish deep extensor abdominal muscle (DEAM). GABA was applied in pulses to outside-out patches of muscle membrane, and, based on the dose-response of the peak currents and of their rise times, a linear model with five binding steps has been proposed. Evaluation of the single-channel kinetics indicated at least three open states. Two of them originate most probably from the fully liganded receptor state and are grouped in mixed bursts due to their different life times. The third one appears independently, outside the bursts, and originates from a lower liganded receptor state. Simulations of the dose-responses and the open time distributions with this model led to a set of rate constants which generated relatively optimal fits.