Referenced in: none

Automatically associated channels: Kir2.3

Title: A heterogeneous electrophysiological profile of bone marrow-derived mast cells.

Authors: M Kuno, T Shibata, J Kawawaki, I Kyogoku

Journal, date & volume: J. Membr. Biol., 1995 Jan , 143, 115-22

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/7731031

Abstract
Electrophysiological properties of mouse bone marrow-derived mast cells (BMMC) were studied under the whole-cell clamp configuration. About one third of the cells were quiescent, but others expressed either inward or outward currents. Inwardly rectifying (IR) currents were predominant in 14% of the cells, and outwardly rectifying (OR) currents in 24%. The rest (22%) of the cells exhibited both inward and outward currents. The IR currents were eliminated by 1 mM Ba2+, and were partially inhibited by 100 microM quinidine. The reversal potential was dependent on extracellular K+, thereby indicating that K+ mediated the IR currents. The negative conductance region was seen at potentials positive to EK. The OR currents did not apparently depend on the extracellular K+ concentration, but were reduced by lowering the extracellular Cl- concentration. The OR currents were partially blocked by 1 mM Ba2+, and were further blocked by a Cl- channel blocker, 4,4'-diisothiocyano-2,2'-stilbenedisulfonate (DIDS). In addition, the reversal potential of the OR currents was positively shifted by decreasing the ratio of external and internal Cl- concentrations, suggesting that Cl- was a major ion carrier. In cells exhibiting IR currents, the membrane potential varied among cells and tended to depolarize by elevating the external K+ concentration. In cells with OR currents, the resting potential was hyperpolarized in association with an increase in conductance.(ABSTRACT TRUNCATED AT 250 WORDS)