Referenced in: none

Automatically associated channels: Kv2.1 , Slo1

Title: Selective loss of slow and enhancement of fast Na+ currents in cutaneous afferent dorsal root ganglion neurones following axotomy.

Authors: M A Rizzo, J D Kocsis, S G Waxman

Journal, date & volume: Neurobiol. Dis., 1995 Apr , 2, 87-96

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8980012

Abstract
Voltage-dependent Na+ currents were recorded via patch-clamp from identified adult rat lumbar cutaneous dorsal root ganglion (DRG) neurones using whole-cell and bleb-patch-clamp configurations. Na+ currents in DRG neurones studied 18 days after sciatic nerve ligation were compared with those in control neurones. Control neurones tended to have a singular kinetically slow Na+ current or net Na+ current suggestive of two kinetic varieties of channel in a single neurone. Three changes occurred following axotomy: (1) the peak Na+ current increased significantly, (2) kinetically slow TTX-resistant Na+ current, which predominated in controls, was significantly attenuated or lacking altogether, and (3) a singular, TTX-sensitive kinetically fast form of Na+ current predominated. These findings suggest that as part of the response to axonal injury, DRG neurones increase Na+ channel biosynthesis and/or modify preexisting channels such that their kinetics are accelerated. The results are consistent with the idea that axotomized DRG neurones become hyperexcitable due to the emergence of a high density of kinetically fast, pharmacologically distinguishable, Na+ channels in the membrane.