Referenced in: none

Automatically associated channels: Kir2.3

Title: Immunochemical characterization and developmental expression of Shaker potassium channels from the nervous system of Drosophila.

Authors: O Rogero, F J Tejedor

Journal, date & volume: J. Biol. Chem., 1995 Oct 27 , 270, 25746-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/7592756

Abstract
We have raised antisera against recombinant peptides expressed from cDNAs fragments common to all splicing variants generated at the Shaker locus of Drosophila and used them as a tool to biochemically characterize these channel proteins. This antisera succeeded in detecting the expression of multiple Shaker potassium channels (Sh Kch), proteins with variable molecular mass (65-85 kDa) and pI (5.5-7). Additionally, for first time, specific Sh proteins of 40-45 kDa most probably corresponding to some of the so-called short Sh cDNAs previously isolated by others have been identified. Using genetic criteria, it has been determined that at least a good part of this variety of proteins is generated by alternative splicing. Developmental experiments show a double wave of Sh Kch channel expression with a first pick at the third instar larvae stage, a minimum at the beginning of puparation, and the highest plateau 36 h after hatching of adult flies. The pattern of Sh splice variants changes dramatically throughout development. A detergent-resistant fraction with about 50% of Sh Kch which seems to be anchored to submembranous structures has been found. Finally, other biochemical properties of Sh Kch, like membrane fractionation and glycosylation, are also described.