Referenced in: none

Automatically associated channels: ClC4

Title: Gating of the voltage-dependent chloride channel CIC-0 by the permeant anion.

Authors: M Pusch, U Ludewig, A Rehfeldt, T J Jentsch

Journal, date & volume: Nature, 1995 Feb 9 , 373, 527-31

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/7845466

Abstract
Chloride channels of the ClC family are important for the control of membrane excitability, cell volume regulation, and possibly transepithelial transport. Although lacking the typical voltage-sensor found in cation channels, gating of ClC channels is clearly voltage-dependent. For the prototype Torpedo channel ClC-0 (refs 11-15) we now show that channel opening is strongly facilitated by external chloride. Other less permeable anions can substitute for chloride with less efficiency. ClC-0 conductance shows an anomalous mole fraction behaviour with Cl-/NO3- mixtures, suggesting a multi-ion pore. Gating shows a similar anomalous behaviour, tightly linking permeation to gating. Eliminating a positive charge at the cytoplasmic end of domain D12 changes kinetics, concentration dependence and halide selectivity of gating, and alters pore properties such as ion selectivity, single-channel conductance and rectification. Taken together, our results strongly suggest that in these channels voltage-dependent gating is conferred by the permeating ion itself, acting as the gating charge.