Referenced in: none

Automatically associated channels: Kv1.4

Title: Molecular properties of voltage-gated K+ channels.

Authors: J O Dolly, D N Parcej

Journal, date & volume: J. Bioenerg. Biomembr., 1996 Jun , 28, 231-53

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8807399

Abstract
Subfamilies of voltage-activated K+ channels (Kv1-4) contribute to controlling neuron excitability and the underlying functional parameters. Genes encoding the multiple alpha subunits from each of these protein groups have been cloned, expressed and the resultant distinct K+ currents characterized. The predicted amino acid sequences showed that each alpha subunit contains six putative membrane-spanning alpha-helical segments (S1-6), with one (S4) being deemed responsible for the channels' voltage sensing. Additionally, there is an H5 region, of incompletely defined structure, that traverses the membrane and forms the ion pore; residues therein responsible for K+ selectively have been identified. Susceptibility of certain K+ currents produced by the Shaker-related subfamily (Kv1) to inhibition by alpha-dendrotoxin has allowed purification of authentic K+ channels from mammalian brain. These are large (M(r) approximately 400 kD), octomeric sialoglycoproteins composed of alpha and beta subunits in a stoichiometry of (alpha)4(beta)4, with subtypes being created by combinations of subunit isoforms. Subsequent cloning of the genes for beta 1, beta 2 and beta 3 subunits revealed novel sequences for these hydrophilic proteins that are postulated to be associated with the alpha subunits on the inner side of the membrane. Coexpression of beta 1 and Kv1.4 subunits demonstrated that this auxiliary beta protein accelerates the inactivation of the K+ current, a striking effect mediate by an N-terminal moiety. Models are presented that indicate the functional domains pinpointed in the channel proteins.