Channelpedia

PubMed 8702582


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.1 , Slo1



Title: Expression of Kv1.1 delayed rectifier potassium channels in Lec mutant Chinese hamster ovary cell lines reveals a role for sialidation in channel function.

Authors: W B Thornhill, M B Wu, X Jiang, X Wu, P T Morgan, J F Margiotta

Journal, date & volume: J. Biol. Chem., 1996 Aug 9 , 271, 19093-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8702582


Abstract
Kv1.1 potassium (K+) channels contain significant amounts of negatively charged sialic acids. To examine the role of sialidation in K+ channel function, Chinese hamster ovary cell lines deficient in glycosylation (Lec mutants) were transfected with rat brain Kv1.1 cDNA. The K+ channel was functionally expressed in all cell lines, but the voltage dependence of activation (V1/2) was shifted to more positive voltages and the activation kinetics were slower in the mutant cell lines compared with control. A similar positive shift in V1/2 was recorded in control cells expressing Kv1.1 following treatment with sialidase or by raising extracellular Ca2+. In contrast, these treatments had little or no effect on the Lec mutants, which indicates that channel sialic acids appear to be the negative surface charges sensitive to Ca2+. The data suggest that sialic acid addition modifies Kv1.1 channel function, possibly by influencing the local electric field detected by its voltage sensor, but that these carbohydrates are not required for cell surface expression.