Referenced in: none

Automatically associated channels: Kir6.2

Title: Developmental regulation of ENaC subunit mRNA levels in rat kidney.

Authors: V M Vehaskari, J M Hempe, J Manning, D H Aviles, M C Carmichael

Journal, date & volume: Am. J. Physiol., 1998 Jun , 274, C1661-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9611132

Abstract
To assess the role of distal nephron apical Na channel (ENaC) gene expression in Na wasting by the immature kidney, ENaC alpha-, beta-, and gamma-subunit mRNA levels were examined in the rat by RT-PCR. In microdissected nephron segments, all three ENaC subunit mRNAs were detected in the distal convoluted tubule, connecting tubule, cortical collecting duct, and outer medullary collecting duct. The inner medullary collecting duct and all other nephron segments were consistently negative. The mRNA levels were quantified in kidneys at different developmental stages by multiplex RT-PCR with "primer dropping," with endoplasmic reticulum-specific cyclophilin mRNA as an internal standard. All three ENaC mRNA levels were low or undetectable on gestational day 16 and only slightly higher 3 days before birth. A sharp rise was observed between 3 days before and 1-3 days after birth; the levels at postnatal days 1-3 were already similar to those of adult kidneys. The results suggest that ENaC subunit gene expression is not a limiting factor in the full-term newborn rat kidney, but low levels of expression may limit distal Na absorption in more immature kidneys, such as those of very premature human infants.