Referenced in: none

Automatically associated channels: Kv10.1

Title: Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line.

Authors: C E Stansfeld, J Röper, J Ludwig, R M Weseloh, S J Marsh, D A Brown, O Pongs

Journal, date & volume: Proc. Natl. Acad. Sci. U.S.A., 1996 Sep 3 , 93, 9910-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/8790430

Abstract
We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of calcium-dependent kinases and phosphatases. The effect of calcium on r-eag channel activity was studied in inside-out membrane patches. Calcium inhibited r-eag channel activity with a mean IC50 of 67 nM. Activation of muscarinic receptors, generating calcium oscillations in the transfected cells, induced a synchronous inhibition of r-eag mediated outward currents. This shows that calcium can mediate r-eag current inhibition following muscarinic receptor activation. The data indicate that r-eag channels are calcium-inhibitable voltage-activated potassium channels.