Referenced in: none

Automatically associated channels: ClC1 , ClC2 , ClC3 , ClC4

Title: Single-cell RT-PCR demonstrates expression of voltage-dependent chloride channels (ClC-1, ClC-2 and ClC-3) in outer hair cells of rat cochlea.

Authors: E Kawasaki, N Hattori, E Miyamoto, T Yamashita, C Inagaki

Journal, date & volume: Brain Res., 1999 Aug 14 , 838, 166-70

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/10446329

Abstract
We investigated whether voltage-dependent chloride channels (ClC-1, ClC-2 and ClC-3) are expressed in outer hair cells (OHCs) of rat cochlea using a single-cell reverse transcription-polymerase chain reaction (RT-PCR) technique. The OHCs were isolated from rat cochlea and the cytoplasm of each OHC was suctioned into a glass pipette containing RT-PCR reaction buffer with RNase inhibitor. RT-PCR revealed the presence of transcripts of ClC-1, ClC-2 and ClC-3, which were verified by DNA sequencing. The possible roles of these chloride channels in OHCs are discussed.