Referenced in: none

Automatically associated channels: Kv2.1 , Kv7.1 , Slo1

Title: Adult KCNE1-knockout mice exhibit a mild cardiac cellular phenotype.

Authors: F Charpentier, J Merot, D Riochet, H Le Marec, D Escande

Journal, date & volume: Biochem. Biophys. Res. Commun., 1998 Oct 29 , 251, 806-10

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9790991

Abstract
The KCNE1 gene encodes a channel regulator IsK which in association with the KvLQT1 K+ channel protein determines the slow component of the cardiac delayed rectifier current. We have investigated the cellular electrophysiological characteristics of adult KCNE1-knockout mouse hearts by means of the standard microelectrode technique. Action potential parameters from the ventricular endocardium of KCNE1 -/- mice were indistinguishable from those of KCNE1 +/+ animals. In particular, KCNE1 -/- hearts did not exhibit prolonged repolarization. E-4031, a specific blocker of erg K+ channels consistently prolonged repolarization in KCNE1 +/+ but not in KCNE1 -/- hearts. By contrast, the chromanol compound 293B, a specific blocker of KvLQT1 K+ channel produced comparable effects on repolarization in KCNE1 -/- and KCNE1 +/+ mice. We conclude that invalidation of the mouse KCNE1 gene by homologous recombination leads to a mild cardiac phenotype at the cellular level.