Referenced in: none

Automatically associated channels: ClC4 , ClC5

Title: Tissue distribution and subcellular localization of the ClC-5 chloride channel in rat intestinal cells.

Authors: A Vandewalle, F Cluzeaud, K C Peng, M Bens, A Lüchow, W Günther, T J Jentsch

Journal, date & volume: Am. J. Physiol., Cell Physiol., 2001 Feb , 280, C373-81

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11208533

Abstract
ClC-5 is the Cl- channel that is mutated in Dent's disease, an X-chromosome-linked disease characterized by low molecular weight proteinuria, hypercalciuria, and kidney stones. It is predominantly expressed in endocytically active renal proximal cells. We investigated whether this Cl- channel could also be expressed in intestinal tissues that have endocytotic machinery. ClC-5 mRNA was detected in the rat duodenum, jejunum, ileum, and colon. Western blot analyses revealed the presence of the 83-kDa ClC-5 protein in these tissues. Indirect immunofluorescence studies showed that ClC-5 was mainly concentrated in the cytoplasm above the nuclei of enterocytes and colon cells. ClC-5 partially colocalized with the transcytosed polymeric immunoglobulin receptor but was not detectable together with the brush-border-anchored sucrase isomaltase. A subfractionation of vesicles obtained by differential centrifugation showed that ClC-5 is associated with the vacuolar 70-kDa H+-ATPase and the small GTPases rab4 and rab5a, two markers of early endosomes. Thus these results indicate that ClC-5 is present in the small intestine and colon of rats and suggest that it plays a role in the endocytotic pathways of intestinal cells.