Referenced in: none

Automatically associated channels: Kv11.1

Title: Development and evaluation of high throughput functional assay methods for HERG potassium channel.

Authors: W Tang, J Kang, X Wu, D Rampe, L Wang, H Shen, Z Li, D Dunnington, T Garyantes

Journal, date & volume: , 2001 Oct , 6, 325-31

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11689132

Abstract
Three functional hERG channel assay methods have been developed and evaluated. The methods were tested against five known hERG channel inhibitors: dofetilide, terfenadine (Seldane), sertindole (Serdolect), astemizole (Hismanal), and cisapride (Propulsid). The DiBAC4(3)-based assays were found to be the most economical but had high false-hit rates as a result of the interaction of dye with the test compounds. The membrane potential dye assay had fewer color-quenching problems but was expensive and still gave false hits. The nonradioactive Rb+ efflux assay was the most sensitive of all the assays evaluated and had the lowest false-hit rate.