Referenced in: none

Automatically associated channels: Kir2.3

Title: Genomic and functional characteristics of novel human pancreatic 2P domain K(+) channels.

Authors: C Girard, F Duprat, C Terrenoire, N Tinel, M Fosset, G Romey, M Lazdunski, F Lesage

Journal, date & volume: Biochem. Biophys. Res. Commun., 2001 Mar 23 , 282, 249-56

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11263999

Abstract
We isolated three novel 2P domain K(+) channel subunits from human. The first two subunits, TALK-1 and TALK-2, are distantly related to TASK-2. Their genes form a tight cluster of 25 kb on chromosome 6p21.1-p21.2. The corresponding channels produce quasi-instantaneous and non-inactivating currents that are activated at alkaline pHs. These currents are sensitive to Ba(2+), quinine, quinidine, chloroform, halothane, and isoflurane but are not affected by TEA, 4-AP, Cs(+), arachidonic acid, hypertonic solutions, agents activating protein kinases C and A, changes of internal Ca(2+) concentrations, and by activation of G(i) and G(q) proteins. TALK-1 is exclusively expressed in the pancreas. TALK-2 is mainly expressed in the pancreas, but is also expressed at a lower level in liver, placenta, heart, and lung. We also cloned a third subunit, named hTHIK-2 which is present in many tissues with high levels again in the pancreas but which could not be functionally expressed.