Referenced in: none

Automatically associated channels: Kv10.1 , Slo1

Title: Modulation of renal type IIa Na+/Pi cotransporter kinetics by the arginine modifier phenylglyoxal.

Authors: I C Forster, K Köhler, G Stange, J Biber, H Murer

Journal, date & volume: J. Membr. Biol., 2002 May 15 , 187, 85-96

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/12029367

Abstract
The effects of the arginine-modifying reagent phenylglyoxal on the kinetics of the type IIa Na + /Pi cotransporter expressed in Xenopus, oocytes were studied by means of 32Pi uptake and electrophysiology. Phenylglyoxal incubation induced up to 60% loss of cotransport function but only marginally altered the Na+-leak. Substrate activation and pH dependency remained essentially unaltered, whereas the voltage dependency of Pi-induced change in electrogenic response was significantly reduced. Presteady-state charge movements were suppressed and the equilibrium charge distribution was shifted slightly towards hyperpolarizing potentials. Charge movements in the absence of external Na+ were also suppressed, which indicated that the empty-carrier kinetics were modified. These effects were incorporated into an ordered alternating access model for NaPi-IIa, whereby the arginine modification by phenylglyoxal was modeled as altered apparent electrical distances moved by mobile charges, together with a slower rate of translocation of the electroneutral, fully loaded carrier.