Channelpedia

PubMed 24953032


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Nav1 , Nav1.1



Title: Generation of the SCN1A epilepsy mutation in hiPS cells using the TALEN technique.

Authors: Wanjuan Chen, Jingxin Liu, Longmei Zhang, Huijuan Xu, Xiaogang Guo, Sihao Deng, Lipeng Liu, Daiguan Yu, Yonglong Chen, Zhiyuan Li

Journal, date & volume: Sci Rep, 2014 , 4, 5404

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24953032


Abstract
Human induced pluripotent stem cells (iPSC) can be used to understand the pathological mechanisms of human disease. These cells are a promising source for cell-replacement therapy. However, such studies require genetically defined conditions. Such genetic manipulations can be performed using the novel Transcription Activator-Like Effector Nucleases (TALENs), which generate site-specific double-strand DNA breaks (DSBs) with high efficiency and precision. Combining the TALEN and iPSC methods, we developed two iPS cell lines by generating the point mutation A5768G in the SCN1A gene, which encodes the voltage-gated sodium channel Nav1.1 α subunit. The engineered iPSC maintained pluripotency and successfully differentiated into neurons with normal functional characteristics. The two cell lines differ exclusively at the epilepsy-susceptibility variant. The ability to robustly introduce disease-causing point mutations in normal hiPS cell lines can be used to generate a human cell model for studying epileptic mechanisms and for drug screening.