Referenced in: none

Automatically associated channels: Slo1 , TRP , TRPV , TRPV2

Title: Activation of TRPV2 and BKCa channels by the LL-37 enantiomers stimulates calcium entry and migration of cancer cells.

Authors: Audrey Gambade, Sami Zreika, Maxime Guéguinou, Igor Chourpa, Gaëlle Fromont, Ana Maria Bouchet, Julien Burlaud-Gaillard, Marie Potier-Cartereau, Sébastien Roger, Vincent Aucagne, Stéphan Chevalier, Christophe Vandier, Caroline Goupille, Günther Weber

Journal, date & volume: Oncotarget, 2016 Mar 16 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26993604

Abstract
Expression of the antimicrobial peptide hCAP18/LL-37 is associated to malignancy in various cancer forms, stimulating cell migration and metastasis. We report that LL-37 induces migration of three cancer cell lines by activating the TRPV2 calcium-permeable channel and recruiting it to pseudopodia through activation of the PI3K/AKT pathway. Ca2+ entry through TRPV2 cooperated with a K+ efflux through the BKCa channel. In a panel of human breast tumors, the expression of TRPV2 and LL-37 was found to be positively correlated. The D-enantiomer of LL-37 showed identical effects as the L-peptide, suggesting that no binding to a specific receptor was involved. LL-37 attached to caveolae and pseudopodia membranes and decreased membrane fluidity, suggesting that a modification of the physical properties of the lipid membrane bilayer was the underlying mechanism of its effects.