Channelpedia

PubMed 24430351


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Nav1 , Nav1.2



Title: PKA phosphorylation reshapes the pharmacological kinetics of BmK AS, a unique site-4 sodium channel-specific modulator.

Authors: Z R Liu, H Zhang, J Q Wu, J J Zhou, Y H Ji

Journal, date & volume: Sci Rep, 2014 , 4, 3721

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24430351


Abstract
Although modulation of the activity of voltage-gated sodium channels (VGSCs) by protein kinase A (PKA) phosphorylation has been investigated in multiple preparations, the pharmacological sensitivity of VGSCs to scorpion toxins after PKA phosphorylation has rarely been approached. In this study, the effects of BmK AS, a sodium channel-specific modulator from Chinese scorpion Buthus martensi Karsch, on the voltage-dependent activation and inactivation of Nav1.2 were examined before and after PKA activation. After PKA phosphorylation, the pattern of dose-dependent modulation of BmK AS, on both Nav1.2α and Nav1.2 (α + β1) was reshaped. Meanwhile, the shifts in voltage-dependency of activation and inactivation induced by BmK AS were attenuated. The results suggested that PKA might play a role in different patterns how β-like toxins such as BmK AS modulate gating properties and peak currents of VGSCs.