Referenced in: none

Automatically associated channels: TRP , TRPA , TRPA1

Title: Formalin evokes calcium transients from the endoplasmatic reticulum.

Authors: Michael J M Fischer, Kailey J Soller, Susanne K Sauer, Joanna Kalucka, Gianluigi Veglia, Peter W Reeh

Journal, date & volume: PLoS ONE, 2015 , 10, e0123762

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/25875358

Abstract
The formalin test is the most widely used behavioral screening test for analgesic compounds. The cellular mechanism of action of formaldehyde, inducing a typically biphasic pain-related behavior in rodents is addressed in this study. The chemoreceptor channel TRPA1 was suggested as primary transducer, but the high concentrations used in the formalin test elicit a similar response in TRPA1 wildtype and knockout animals. Here we show that formaldehyde evokes a dose-dependent calcium release from intracellular stores in mouse sensory neurons and primary keratinocytes as well as in non-neuronal cell lines, and independent of TRPA1. The source of calcium is the endoplasmatic reticulum and inhibition of the sarco/endoplasmic reticulum calcium-ATPase has a major contribution. This TRPA1-independent mechanism may underlie formaldehyde-induced pan-neuronal excitation and subsequent inflammation.