Referenced in: none

Automatically associated channels: Kv2.1

Title: Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride.

Authors: Jing Zou, Ilmari Pyykkö

Journal, date & volume: Ann. Otol. Rhinol. Laryngol., 2015 Jul 29 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/26229013

Abstract
To evaluate the efficacy of the novel method for the targeted delivery of Mn(++) to the inner ear and monitor calcium metabolism activity in the inner ear.Dynamic signal changes of Mn(++) in the rat inner ear were followed using T1-weighted magnetic resonance imaging (MRI) after administration of 2.5 µl MnCl₂(500 mM) to the medial wall of the middle ear cavity.Mn(++) passed through both the oval and round windows and distributed in the perilymphatic compartments, where it formed bright sharp lines along the fluid-cellular borders 12 minutes post administration and entered the endolymph sufficiently after 45 minutes. After 6 hours, the distribution of Mn(++) shifted from a fluid-dominant pattern to a cell-dominant pattern. Mn(++) concentrated in the area of the basilar membrane, periphery process, and soma of the spiral ganglion on day 2; became more distinguishable on day 4; declined on day 8; and remained detectable for 16 days post administration.The novel targeted delivery method efficiently introduced Mn(++) into the inner ear. The dynamic distribution pattern of Mn(++) in the inner ear shown by MRI indicates that this method can be used to monitor calcium metabolism activity in the inner ear.