Referenced in: none

Automatically associated channels: Kir6.2 , TRP , TRPC , TRPC1 , TRPC5 , TRPC6

Title: Expression and localization of TRPC proteins in rat ventricular myocytes at various developmental stages.

Authors: Yu Jiang, Haixia Huang, Ping Liu, Hua Wei, Haiyan Zhao, Ying Feng, Wei Wang, Weizhen Niu

Journal, date & volume: Cell Tissue Res., 2014 Jan , 355, 201-12

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24146259

Abstract
Growing evidence indicates that transient receptor potential canonical (TRPC) channels play important roles in various Ca(2+)-mediated physiological and pathophysiological processes, including development. Many types of TRPC proteins are expressed in the heart. However, limited data are available comparing the expression and localization among TRPC proteins in the ventricular myocyte at various developmental stages. Our purpose is to investigate the expression and localization profile of TRPC proteins in ventricular myocytes of fetal (18.5 days), neonatal (< 24 h after birth) and adult (8 week old) rats. Western blotting, immunofluorescence and confocal laser scanning microscopy were employed. TRPC1/3-6 proteins were expressed in the rat ventricle throughout the three developmental stages. The expression profile of TRPC1/3/4 in the ventricle followed an upward trend from the fetus to the adult. By contrast, TRPC6 in the ventricle was expressed at the highest level in the fetal group and was sharply down-regulated immediately after birth. TRPC5 expression in the ventricle did not change significantly during the three stages. TRPC1/3/5/6 proteins were localized to the T-tubule and TRPC1/3/4/6 to intercalated disks in adult myocytes. The wide spatiotemporal overlap and dynamic regulation of TRPC expression in ventricular myocytes indicates potential complex combinations and redundancy of native TRPC proteins in the heart and gives important clues for further investigations into the exact subunit compositions and functional properties of native TRPC channels in the heart.