Channelpedia

PubMed 24507595


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.2



Title: The open gate of the K(V)1.2 channel: quantum calculations show the key role of hydration.

Authors: Alisher M Kariev, Philipa Njau, Michael E Green

Journal, date & volume: Biophys. J., 2014 Feb 4 , 106, 548-55

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24507595


Abstract
The open gate of the Kv1.2 voltage-gated potassium channel can just hold a hydrated K(+) ion. Quantum calculations starting from the x-ray coordinates of the channel confirm this, showing little change from the x-ray coordinates for the protein. Water molecules not in the x-ray coordinates, and the ion itself, are placed by the calculation. The water molecules, including their orientation and hydrogen bonding, with and without an ion, are critical for the path of the ion, from the solution to the gate. A sequence of steps is postulated in which the potential experienced by the ion in the pore is influenced by the position of the ion. The gate structure, with and without the ion, has been optimized. The charges on the atoms and bond lengths have been calculated using natural bond orbital calculations, giving K(+) ~0.77 charges, rather than 1.0. The PVPV hinge sequence has been mutated in silico to PVVV (P407V in the 2A79 numbering). The water structure around the ion becomes discontinuous, separated into two sections, above and below the ion. PVPV conservation closely relates to maintaining the water structure. Finally, these results have implications concerning gating.