Referenced in: none

Automatically associated channels: TRP , TRPM , TRPM2

Title: Role of TRPM2 cation channels in dorsal root ganglion of rats after experimental spinal cord injury.

Authors: Mustafa Naziroglu, Abdulhadi Cihangir Uğuz, Özgür Ismailoğlu, Bilal Çiğ, Cemil Ozgül, Muhammed Borcak

Journal, date & volume: Muscle Nerve, 2013 Dec , 48, 945-50

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23512594

Abstract
We sought to determine the contribution of oxidative stress-dependent activation of TRPM2 and L-type voltage-gated Ca(2+) channels (VGCC) in dorsal root ganglion (DRG) neurons of rats after spinal cord injury (SCI).The rats were divided into 4 groups: control; sham control; SCI; and SCI+nimodipine groups. The neurons of the SCI groups were also incubated with non-specific TRPM2 channel blockers, 2-aminoethoxydiphenylborate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA), before H2 O2 stimulation.The [Ca(2+) ]i concentrations were higher in the SCI group than in the control groups, although their concentrations were decreased by nimodipine and 2-APB. The H2 O2 -induced TRPM2 current densities in patch-clamp experiments were decreased by ACA and 2-APB incubation. In the nimodipine group, the TRPM2 channels of neurons were not activated by H2 O2 or cumene hydroperoxide.Increased Ca(2+) influx and currents in DRG neurons after spinal injury indicated TRPM2 and voltage-gated Ca(2+) channel activation.