Referenced in: none

Automatically associated channels: Slo1

Title: Tracking single-particle dynamics via combined optical and electrical sensing.

Authors: Naoya Yukimoto, Makusu Tsutsui, Yuhui He, Hirofumi Shintaku, Shoji Tanaka, Satoyuki Kawano, Tomoji Kawai, Masateru Taniguchi

Journal, date & volume: Sci Rep, 2013 , 3, 1855

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23685401

Abstract
While fluorescent imaging has been extensively used for single-particle tracking, temporal and spatial resolution of the wide-field microscopy technology is not satisfactory for investigating fast-moving features. Here we report a method for probing nanometer-scale motion of an individual particle through a microstructured channel at sub-MHz by combining a resistive pulse technique to the optical sensing. We demonstrate unambiguous discriminations of translocation and non-translocation events inferred from spike-like electrical signals by fluorescence images captured synchronously to ionic current measurements. We also find a trajectory-dependent translocation dynamics of voltage-driven single-particles through a microchannel. This electrical/optical approach may find applications in sensor technologies based on micro- and nano-electromechanical systems.