Referenced in: none

Automatically associated channels: Kv11.1

Title: Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers.

Authors: Mark S Friddin, Natalie P Smithers, Maïwenn Beaugrand, Isabelle Marcotte, Philip T F Williamson, Hywel Morgan, Maurits R R de Planque

Journal, date & volume: Analyst, 2013 Nov 12 , 138, 7294-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24162163

Abstract
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.