Referenced in: none

Automatically associated channels: HCN2

Title: [Adipose tissue-derived adult stem cells transfected with the gene of hyperpolarization-activated cyclic nucleotide-gated ion channel 2 differentiated into pacemaker-like cells].

Authors: Yong Li, Bingong Li, Zhe Li, Jian Zhang, Minghui Zeng

Journal, date & volume: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2013 Sep , 29, 901-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/24011147

Abstract
To transfect rat adipose tissue-derived adult stem cells (ADSCs) with the pacemaker gene of human hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (hHCN2), and observe its feasibility of functioning as pacemaker cells.First of all, healthy ADSCs were harvested and transfected with hHCN2 gene. Thereafter, we detected the expression of hHCN2 gene and protein by quantitative real-time PCR (qRT-PCR), Western blot analysis and immunofluorescence cytochemistry and measured the electrophysiological properties and recorded the inward currents using patch clamp techniques. In addition, we mixed and co-cultured the transfected ADSCs and rat cadiomyocytes to observe the influence on the spontaneous beating rate of cadiomyocytes.Compared with the control ADSCs, the transfected ADSCs expressed the higher level of hHCN2 gene and protein(P<0.05), and they could produce pacemaker current. When co-cultured with rat ventricular myocytes, they could accelerate the spontaneous beating rate of the ventricular myocytes(P<0.05).After transfected with hHCN2 gene, ADSCs can work as pacemaker-like cells, which provides materials for the research of biological pacemakers.