Referenced in: none

Automatically associated channels: Slo1

Title: PET imaging of lung inflammation with [18F]FEDAC, a radioligand for translocator protein (18 kDa).

Authors: Akiko Hatori, Joji Yui, Tomoteru Yamasaki, Lin Xie, Katsushi Kumata, Masayuki Fujinaga, Yuichiro Yoshida, Masanao Ogawa, Nobuki Nengaki, Kazunori Kawamura, Toshimitsu Fukumura, Ming-Rong Zhang

Journal, date & volume: PLoS ONE, 2012 , 7, e45065

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22984611

Abstract
The translocator protein (18 kDa) (TSPO) is highly expressed on the bronchial and bronchiole epithelium, submucosal glands in intrapulmonary bronchi, pneumocytes and alveolar macrophages in human lung. This study aimed to perform positron emission tomography (PET) imaging of lung inflammation with [(18)F]FEDAC, a specific TSPO radioligand, and to determine cellular sources enriching TSPO expression in the lung.An acute lung injury model was prepared by intratracheal administration of lipopolysaccharide (LPS) to rat. Uptake of radioactivity in the rat lungs was measured with small-animal PET after injection of [(18)F]FEDAC. Presence of TSPO was examined in the lung tissue using Western blot and immunohistochemical assays.The uptake of [(18)F]FEDAC increased in the lung with the progress of inflammation by treatment with LPS. Pretreatment with a TSPO-selective ligand PK11195 showed a significant decrease in the lung uptake of [(18)F]FEDAC due to competitive binding to TSPO. TSPO expression was elevated in the inflamed lung section and its level responded to the [(18)F]FEDAC uptake and severity of inflammation. Increase of TSPO expression was mainly found in the neutrophils and macrophages of inflamed lungs.From this study we conclude that PET with [(18)F]FEDAC may be a useful tool for imaging TSPO expression and evaluating progress of lung inflammation. Study on human lung using [(18)F]FEDAC-PET is promising.