Referenced in: none

Automatically associated channels: TRP

Title: Transient receptor potential N (TRPN1) from Xenopus interacts with the penta-EF-hand protein peflin.

Authors: Dominik Wiemuth, Lena van de Sandt, Rudolf Herr, Stefan Gründer

Journal, date & volume: FEBS Lett., 2012 Dec 14 , 586, 4276-81

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23159937

Abstract
TRPN1 is a candidate mechanotransduction channel in Drosophila and Caenorhabditis elegans, also present in hair cells of lower vertebrates. At its N-terminal cytoplasmic tail it contains 28 ankyrin repeats. We performed a yeast two-hybrid screen with the N-terminal ankyrin repeats of Xenopus TRPN1 as bait and identified the Penta-EF-hand protein peflin as a putative interaction partner. We confirmed this interaction by GST pulldown assays and by co-localization in an epithelial cell model. Collectively, our study identifies peflin as an interaction partner of TRPN1 in vitro.