Referenced in: none

Automatically associated channels: Kir6.2

Title: [Actions of Syn-1A on blocking the activation of K(ATP) channel induced by acidic pH].

Authors: Jun-li Ma, Guo-qing Zhuang, Yan-xia Luo, Fen Liu, Yan He

Journal, date & volume: Zhongguo Ying Yong Sheng Li Xue Za Zhi, 2008 May , 24, 141-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21141510

Abstract
To investigate the action and mechanism of Syn-1A in reversing the activation of K(ATP) channel induced by weak acidic pH.The patches excised from Kir6.2/SUR2A expressing HEK-293 cells were used to establish inside-out configuration. To examine the actions of weak acidic pH in activation of the channel and the reverse action of Syn-1A on it, the inside-out patches were continuously perfused with the solution of pH from 7.4, 7.0, 6.8, 6.5 to 6.0 with or without Syn-1A. In vitro binding was employed to study the influence of different pH to the binding of Syn-1A to SUR2A subunit.Syn-1A blocked pH 6.5, 6.8 and 7.0 induced activation of the channel, and Syn-1A binding to SUR2A were increased by reducing pH from 7.4 to 6.0.Syn-1A would assert some inhibition of the KATP channels, which might temper the fluctuation of acidic pH-induced K(ATP) channel opening that could induce fatal re-entrant arrhythmias.