Channelpedia

PubMed 22949689


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClvC4 , Kir2.3



Title: Fluoride resistance and transport by riboswitch-controlled CLC antiporters.

Authors: Randy B Stockbridge, Hyun-Ho Lim, Renee Otten, Carole Williams, Tania Shane, Zasha Weinberg, Christopher Miller

Journal, date & volume: Proc. Natl. Acad. Sci. U.S.A., 2012 Sep 18 , 109, 15289-94

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22949689


Abstract
A subclass of bacterial CLC anion-transporting proteins, phylogenetically distant from long-studied CLCs, was recently shown to be specifically up-regulated by F(-). We establish here that a set of randomly selected representatives from this "CLC(F)" clade protect Escherichia coli from F(-) toxicity, and that the purified proteins catalyze transport of F(-) in liposomes. Sequence alignments and membrane transport experiments using (19)F NMR, osmotic response assays, and planar lipid bilayer recordings reveal four mechanistic traits that set CLC(F) proteins apart from all other known CLCs. First, CLC(F)s lack conserved residues that form the anion binding site in canonical CLCs. Second, CLC(F)s exhibit high anion selectivity for F(-) over Cl(-). Third, at a residue thought to distinguish CLC channels and transporters, CLC(F)s bear a channel-like valine rather than a transporter-like glutamate, and yet are F(-)/H(+) antiporters. Finally, F(-)/H(+) exchange occurs with 1:1 stoichiometry, in contrast to the usual value of 2:1.