Referenced in: none

Automatically associated channels: Slo1

Title: Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells.

Authors: Ruiguo Yang, Ning Xi, King Wai Chiu Lai, Kevin Patterson, Hongzhi Chen, Bo Song, Chengeng Qu, Beihua Zhong, Donna H Wang

Journal, date & volume: Nanomedicine, 2012 Nov 22 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/23178285

Abstract
Distinct biochemical, electrochemical and electromechanical coupling processes of pancreatic β-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca(2+) levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca(2+) changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca(2+) levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process.This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process.