Referenced in: none

Automatically associated channels: Kv1.2 , Kv1.4

Title: Electrostatic interaction between inactivation ball and T1-S1 linker region of Kv1.4 channel.

Authors: Zhuo Fan, Xuying Ji, Mingyu Fu, Wanming Zhang, Du Zhang, Zhongju Xiao

Journal, date & volume: Biochim. Biophys. Acta, 2012 Jan , 1818, 55-63

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21996039

Abstract
Inactivation of potassium channels plays an important role in shaping the electrical signaling properties of nerve and muscle cells. The rapid inactivation of Kv1.4 has been assumed to be controlled by a "ball and chain" inactivation mechanism. Besides hydrophobic interaction between inactivation ball and the channel's inner pore, the electrostatic interaction has also been proved to participate in the "ball and chain" inactivation process of Kv1.4 channel. Based on the crystal structure of Kv1.2 channel, the acidic T1-S1 linker is indicated to be a candidate interacting with the positively charged hydrophilic region of the inactivation domain. In this study, through mutating the charged residues to amino acids of opposite polar, we identified the electrostatic interaction between the inactivation ball and the T1-S1 linker region of Kv1.4 channel. Inserting negatively charged peptide at the amino terminal of Kv1.4 channel further confirmed the electrostatic interaction between the two regions.