Referenced in: none

Automatically associated channels: Kir2.3

Title: Lysophosphatidylcholine induces Ca(2+) mobilization in Jurkat human T lymphocytes and CTLL-2 mouse T lymphocytes by different pathways.

Authors: Qi Wang, Yi-Jun Wu

Journal, date & volume: Eur J Pharm Sci, 2011 Dec 18 , 44, 602-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22019492

Abstract
Lysophosphatidylcholine (LPC), an important compound in the immune system, regulates a variety of biological processes. We examined and compared the effect of exogenous LPC on intracellular Ca(2+) overload in human Jurkat CD4+ T lymphocytes and mouse CTLL-2 CD8+ T lymphocytes. LPC caused a dose-dependent intracellular Ca(2+) level ([Ca(2+)](i)) increase in both Jurkat and CTLL-2 lymphocytes. Pretreatment of cells for 5min with 30μM of ruthenium red, a potent ryanodine receptor inhibitor, reduced the LPC-induced Ca(2+) response in both Jurkat and CTLL-2 T lymphocytes. Moreover, pretreatment of cells with 100μM 2-APB for 15min, a cell-permanent IP(3) receptor inhibitor, reduced about two thirds of the LPC induced calcium response in both kinds of cells. However, preincubation of the cells with verapamil, an L-type Ca(2+) channel blocker, did not affect the LPC-induced [Ca(2+)](i) increase in CTLL-2 lymphocytes but inhibited this in Jurkat lymphocytes by 26%. In Ca(2+)-free medium, LPC produced 75.8% of the total [Ca(2+)](i) increase in CTLL-2 lymphocytes and 38% of the total [Ca(2+)](i) increase in Jurkat lymphocytes. These data suggested that the LPC-induced [Ca(2+)](i) increase in human Jurkat and mouse CTLL-2 cell lines occurs via different pathways.