Referenced in: none

Automatically associated channels: Kv3.1

Title: Biophysical properties of Kv3.1 channels in SH-SY5Y human neuroblastoma cells.

Authors: P Friederich, J P Dilger, D Isbrandt, K Sauter, O Pongs, B W Urban

Journal, date & volume: Recept. Channels, 2003 , 9, 387-96

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14698966

Abstract
Biophysical properties of delayed rectifier K channels in the human neuroblastoma SH-SY5Y were established using patch clamp recordings. The whole cell K+ conductance activated at membrane potentials positive to -20 mV. The midpoint of current activation was 9.6 +/- 5.1 mV, the equivalent charge was 3.7 +/-.6. Whole-cell currents inactivated slightly with time constants of 700 ms and 5 s. The K+ currents were sensitive to micromolar concentrations of TEA and 4-aminopyridine. RT-PCR experiments amplified a cDNA fragment specific for human Kv3.1 channels. Activation gating parameters in outside-out patches were shifted by approximately 14 mV in the hyperpolarizing direction.