Referenced in: none

Automatically associated channels: Kir2.3

Title: Pharmaceutical applications of isoelectric focusing on microchip with imaged UV detection.

Authors: Markéta Vlcková, Franka Kalman, Maria A Schwarz

Journal, date & volume: J Chromatogr A, 2008 Feb 15 , 1181, 145-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/18199438

Abstract
For the first time, the application of a commercial Shimadzu microchip electrophoresis system MCE-2010 equipped with an imaging UV detector for isoelectric focusing (IEF) of therapeutic proteins is reported. By proper adjustment of the pH gradient, samples with pI values ranging from 2.85 to 10.3 can be focused to the imaged part of the separation channel. Three therapeutic proteins (hirudin, erythropoietin, and bevacizumab) have been successfully focused on the microchip, and the results have been compared to conventional capillary IEF in terms of peak profile, pI values, and reproducibility.