Referenced in: none

Automatically associated channels: TRP , TRPA , TRPA1 , TRPV , TRPV1

Title: Nociception, neurogenic inflammation and thermoregulation in TRPV1 knockdown transgenic mice.

Authors: Dániel Márton Tóth, Eva Szoke, Kata Bölcskei, Krisztián Kvell, Balázs Bender, Zsuzsanna Bosze, János Szolcsányi, Zoltán Sándor

Journal, date & volume: Cell. Mol. Life Sci., 2011 Aug , 68, 2589-601

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21069423

Abstract
Transgenic mice with a small hairpin RNA construct interfering with the expression of transient receptor potential vanilloid 1 (TRPV1) were created by lentiviral transgenesis. TRPV1 expression level in transgenic mice was reduced to 8% while the expression of ankyrin repeat domain 1 (TRPA1) was unchanged. Ear oedema induced by topical application of TRPV1 agonist capsaicin was completely absent in TRPV1 knockdown mice. Thermoregulatory behaviour in relation to environmental thermopreference (30 vs. 35°C) was slightly impaired in male knockdown mice, but the reduction of TRPV1 function was not associated with enhanced hyperthermia. TRPV1 agonist resiniferatoxin induced hypothermia and tail vasodilatation was markedly inhibited in knockdown mice. In conclusion, shRNA-mediated knock down of the TRPV1 receptor in mice induced robust inhibition of the responses to TRPV1 agonists without altering the expression, gating function or neurogenic oedema provoked by TRPA1 activation. Thermoregulatory behaviour in response to heat was inhibited, but enhanced hyperthermia was not observed.