Referenced in: none

Automatically associated channels: TRP , TRPV , TRPV1

Title: A fast solution switching system with temperature control for single cell measurements.

Authors: Duk-Su Koh, Liangyi Chen, Carmen A Ufret-Vincenty, Seung-Ryoung Jung

Journal, date & volume: J. Neurosci. Methods, 2011 Jul 15 , 199, 35-42

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21536068

Abstract
This article describes a perfusion system for biophysical single cell experiments at the physiological temperature. Our system regulates temperature of test solutions using a small heat exchanger that includes several capillaries. Water circulating inside the heat exchanger warms or cools test solutions flowing inside the capillaries. Temperature-controlled solutions are delivered directly to a single cell(s) through a multibarreled manifold that switches solutions bathing a cell in less than 1s. This solution exchange is optimal for patch clamp, single-cell microamperometry, and microfluorometry experiments. Using this system, we demonstrate that exocytosis from pancreatic β cells and activation of TRPV1 channels are temperature sensitive. We also discuss how to measure local temperature near a single cell under investigation.