Referenced in: none

Automatically associated channels: HCN1

Title: Rapid loss of dendritic HCN channel expression in hippocampal pyramidal neurons following status epilepticus.

Authors: Sangwook Jung, Lindsay N Warner, Julika Pitsch, Albert J Becker, Nicholas P Poolos

Journal, date & volume: J. Neurosci., 2011 Oct 5 , 31, 14291-5

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21976514

Abstract
Epilepsy is associated with loss of expression and function of hyperpolarization-activated, cyclic nucleotide-gated (HCN) ion channels. Previously, we showed that loss of HCN channel-mediated current (I(h)) occurred in the dendrites of CA1 hippocampal pyramidal neurons after pilocarpine-induced status epilepticus (SE), accompanied by loss of HCN1 channel protein expression. However, the precise onset and mechanistic basis of HCN1 channel loss post-SE was unclear, particularly whether it preceded the onset of spontaneous recurrent seizures and could contribute to epileptogenesis or development of the epileptic state. Here, we found that loss of I(h) and HCN1 channel expression began within an hour after SE and involved sequential processes of dendritic HCN1 channel internalization, delayed loss of protein expression, and later downregulation of mRNA expression. We also found that an in vitro SE model reproduced the rapid loss of dendritic I(h), demonstrating that this phenomenon was not specific to in vivo SE. Together, these results show that HCN1 channelopathy begins rapidly and persists after SE, involves both transcriptional and nontranscriptional mechanisms, and may be an early contributor to epileptogenesis.