Referenced in: none

Automatically associated channels: TRP , TRPC , TRPC1

Title: NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli.

Authors: Daria V Ilatovskaya, Vladislav Levchenko, Robert P Ryan, Allen W Cowley, Alexander Staruschenko

Journal, date & volume: Biochem. Biophys. Res. Commun., 2011 May 6 , 408, 242-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21473850

Abstract
Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.