Referenced in: none

Automatically associated channels: Kv10.1

Title: Embryonic Stem Cell-Derived Cardiac Differentiation: Modulation of Differentiation and "Loss-of-Function" Analysis In Vitro.

Authors: A M Wobus, K Guan

Journal, date & volume: Trends Cardiovasc. Med., 1998 Feb , 8, 64-74

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21235914

Abstract
Mouse embryonic stem (ES) cells, when cultivated as embryo-like aggregates, are able to differentiate in vitro into derivatives of all primary germ layers. These include functionally active cardiomyocytes representing ventricle-like, atrium-like, and pacemaker-like cells. During differentiation, a developmentally controlled expression pattern of cardiac-specific genes, proteins, action potentials, ion channels, and receptors is found. This developmental pattern can be modulated in vitro by differentiation factors such as retinoic acid or by "gain-of-function" and "loss-of-function" approaches. The latter strategy was successfully used for the characterization of cardiac phenotypes of β(1) integrin-deficient ES cells after differentiation into the cardiogenic lineage.