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PubMed 20196953


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: ClIC1



Title: [Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines].

Authors: Rong-kuan Li, Jian-wu Tang, Jun Zhang, Shao-qing Wang, Mei Wang, Bo Wang, Yu-hong Zhang

Journal, date & volume: Zhonghua Gan Zang Bing Za Zhi, 2010 Feb , 18, 131-5

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20196953


Abstract
To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.CLIC1 is essential for the proliferation and invasion of Hca-F cells.