Referenced in: none

Automatically associated channels: Slo1

Title: A novel fast mechanism for GPCR-mediated signal transduction--control of neurotransmitter release.

Authors: Yonatan M Kupchik, Ofra Barchad-Avitzur, Jürgen Wess, Yair Ben-Chaim, Itzchak Parnas, Hanna Parnas

Journal, date & volume: J. Cell Biol., 2011 Jan 10 , 192, 137-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21200029

Abstract
Reliable neuronal communication depends on accurate temporal correlation between the action potential and neurotransmitter release. Although a requirement for Ca(2+) in neurotransmitter release is amply documented, recent studies have shown that voltage-sensitive G protein-coupled receptors (GPCRs) are also involved in this process. However, how slow-acting GPCRs control fast neurotransmitter release is an unsolved question. Here we examine whether the recently discovered fast depolarization-induced charge movement in the M(2)-muscarinic receptor (M(2)R) is responsible for M(2)R-mediated control of acetylcholine release. We show that inhibition of the M(2)R charge movement in Xenopus oocytes correlated well with inhibition of acetylcholine release at the mouse neuromuscular junction. Our results suggest that, in addition to Ca(2+) influx, charge movement in GPCRs is also necessary for release control.