Referenced in: none

Automatically associated channels: Kv10.1

Title: Identification of subunits of acetylcholine receptor that interact with a cholesterol photoaffinity probe.

Authors: D S Middlemas, M A Raftery

Journal, date & volume: Biochemistry, 1987 Mar 10 , 26, 1219-23

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/3567168

Abstract
All four subunits of the acetylcholine receptor in membrane vesicles isolated from Torpedo californica have been labeled with [3H]cholesteryl diazoacetate. As this probe incorporates into lipid bilayers analogously to cholesterol, this result indicates that acetylcholine receptor interacts with cholesterol. This investigation also demonstrates that this probe is a useful reagent for studying the interaction of cholesterol with membrane proteins.