Referenced in: none

Automatically associated channels: Kir2.3

Title: Clusters of 43-kDa protein are absent from genetic variants of C2 muscle cells with reduced acetylcholine receptor expression.

Authors: W J LaRochelle, E Ralston, J R Forsayeth, S C Froehner, Z W Hall

Journal, date & volume: Dev. Biol., 1989 Mar , 132, 130-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2645181

Abstract
Genetic variants of the C2 muscle cell line were used to investigate the relation between acetylcholine receptor (AChR) clustering and clustering of the 43-kDa protein. Two variants that express severely reduced amounts of the alpha subunit of the AChR and consequently lack AChR clusters were found also to lack clusters of the 43-kDa protein. The amount of 43-kDa protein in the variants measured by immunoassay was reduced to about one-third the levels found in wild-type cells. The beta subunit of the AChR was reduced to a similar extent. Northern blot analysis showed that neither the 43-kDa protein mRNA nor the beta subunit mRNA was reduced in the variants. Taken together, these results suggest that the amounts of beta subunit and 43-kDa protein may be regulated coordinately by a post-transcriptional mechanism.