Referenced in: none

Automatically associated channels: ClC4

Title: Localization of a putative ClC chloride channel in spinach chloroplasts.

Authors: Enrico Teardo, Erica Frare, Anna Segalla, Veronica De Marco, Giorgio Mario Giacometti, Ildikò Szabò

Journal, date & volume: FEBS Lett., 2005 Sep 12 , 579, 4991-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16115625

Abstract
Seven genes seem to encode for putative ClC chloride channels (AtClC-a to AtClC-g) in Arabidopsis thaliana. Their function and localization is still largely unknown. AtClC-f shares considerable sequence similarity with putative ClC channel proteins from Synechocystis, considered to represent the precursor of chloroplasts. We show by biochemical and mass spectrometry analysis that ClC-f is located in the outer envelope membrane of spinach chloroplasts. Consistent with the plastidial localization of ClC-f, p-chlorophenoxy-acetic acid (CPA) reduces photosynthetic activity and the protein is expressed in etioplasts and chloroplasts but not in root tissue. These findings may represent a step toward the molecular identification of ion channel activities in chloroplast membranes.