Referenced in: none

Automatically associated channels: Kv10.1

Title: Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.

Authors: L P Fernando, Z U Khan, R M McKernan, A L De Blas

Journal, date & volume: J. Neurochem., 1995 Mar , 64, 1305-11

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/7861163

Abstract
The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.